首页 / 院系成果 / 成果详情页

Development of a bioluminescent immunoassay based on Fc-specific conjugated antibody-nanoluciferase immunoreagents for determining aflatoxin B1  期刊论文  

  • 编号:
    0E06015665BCB02C8B328B1DC0B3CD84
  • 作者:
    Du, Yue#[1]Yang, HongMing[1];Zhang, YuMin[1];Ma, Lan[1];Gong, XiaoMing[2];Tang, JinBao*[1]
  • 语种:
    英文
  • 期刊:
    FOOD CHEMISTRY ISSN:0308-8146 2025 年 463 卷 ; JAN 15
  • 收录:
  • 关键词:
  • 摘要:

    Aflatoxin B-1 (AFB(1)) is a potent carcinogen, and is among the most hazardous mycotoxins in agricultural products. Therefore, the development of sensitive and convenient detection methods for AFB(1) is significant for food safety against mycotoxins. Herein, a bioluminescent enzyme immunoassay (BLEIA) was developed for ultrasensitive detection of AFB(1), based on the novel Fc-specific antibody-nanoluciferase (Ab-Nluc) conjugates which were fabricated using an IgG-binding protein-assisted photo-conjugation strategy. In indirect competitive immunoassay format, the proposed BLEIA exhibited the detection limit of 0.0232 ng mL(- 1), which was 37.4-fold lower than that obtained using the classical enzyme-linked immunosorbent assay (ELISA) based on Ab-horseradish peroxidase (Ab-HRP) chemical conjugates (0.868 ng mL(-1)). Meanwhile, the BLEIA exhibited high accuracy and precision. Thus, the proposed Fc-specific Ab-Nluc conjugates-based BLEIA provides an ultrasensitive and reliable method for detecting toxins and has potential for use in food safety monitoring.

  • 推荐引用方式
    GB/T 7714:
    Du Yue,Yang Hong-Ming,Zhang Yu-Min, et al. Development of a bioluminescent immunoassay based on Fc-specific conjugated antibody-nanoluciferase immunoreagents for determining aflatoxin B1 [J].FOOD CHEMISTRY,2025,463.
  • APA:
    Du Yue,Yang Hong-Ming,Zhang Yu-Min,Ma Lan,&Tang Jin-Bao.(2025).Development of a bioluminescent immunoassay based on Fc-specific conjugated antibody-nanoluciferase immunoreagents for determining aflatoxin B1 .FOOD CHEMISTRY,463.
  • MLA:
    Du Yue, et al. "Development of a bioluminescent immunoassay based on Fc-specific conjugated antibody-nanoluciferase immunoreagents for determining aflatoxin B1" .FOOD CHEMISTRY 463(2025).
  • 入库时间:
    10/1/2024 9:39:27 PM
  • 更新时间:
    10/31/2024 9:34:24 PM
浏览次数:4 下载次数:0
浏览次数:4
下载次数:0
打印次数:0
浏览器支持: Google Chrome   火狐   360浏览器极速模式(8.0+极速模式) 
返回顶部